CRL-1661 UMR-106 大鼠骨肉瘤細(xì)胞

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產(chǎn)品名稱(chēng): CRL-1661 UMR-106 大鼠骨肉瘤細(xì)胞
產(chǎn)品型號(hào): CRL-1661 UMR-106
產(chǎn)品廠商: 美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
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CRL-1661 UMR-106 大鼠骨肉瘤細(xì)胞， ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！

CRL-1661 UMR-106 大鼠骨肉瘤細(xì)胞的詳細(xì)介紹

CRL-1661 UMR-106 大鼠骨肉瘤細(xì)胞

ATCC^® Number:

CRL-1661™

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Designations:	UMR-106
Depositors:	AE Bogden
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Rattus norvegicus deposited as Rattus sp.
Morphology:	epithelial
Source:	Organ: bone Strain: Sprague-Dawley Disease: osteosarcoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors:	parathyroid hormone (PTH); 1-25(OH)2D3 (bone resorbing steroid hormone)
Comments:	The UMR-106 cell line is a clonal derivative of a transplantable rat osteosarcoma that had been induced by injection of radiophosphorous (32P). The cells are responsive to PTH, prostaglandins and bone resorbing steroids. The PTH responsiveness of UMR-106 is greater than that of the related cell line UMR-108 (ATCC CRL-1663). Activation of protein kinase C inhibits ATP induced increases in intracellular calcium levels. Both the original sarcoma and the cloned line were developed by T.J. Martin at the University of Sheffield.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References:	21441: Banerjee C, et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. PubMed: 8643513 21887: . Endocrinology of calcium metabolism. Amsterdam: Elsevier/North Holland; 1977. 22331: Atkins D, et al. Rat osteogenic sarcoma cells: modulation of hormone stimulated cyclic AMP production by prostaglandin antagonists and biosynthesis inhibitors. Clin. Exp. Pharmacol. Physiol. 7: 31-44, 1980. PubMed: 6103768 22684: Manolagas SC, Deftos LJ. Cytoreceptor assay for 1,25-dihydroxyvitamin D3: a novel radiometric method based on binding of the hormone to intracellular receptors in vitro. Lancet 2: 401-402, 1980. PubMed: 6105521 22771: Partridge NC, et al. Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Lett. 115: 139-142, 1980. PubMed: 6248375 22847: Ingleton PM, et al. Alkaline phosphatase in serum and tumour of rats bearing a hormone- responsive transplantable osteogenic sarcoma. Eur. J. Cancer 15: 685-691, 1979. PubMed: 292595 22873: Crawford A, et al. Membranes from a transplantable osteogenic sarcoma responsive to parathyroid hormone and prostaglandins: regulation of adenylate cyclase and of hormone metabolism. J. Endocrinol. 77: 213-224, 1978. PubMed: 275436 22874: Atkins D, Martin TJ. Rat osteogenic sarcoma cells:effects of some prostaglandins, their metabolites and analogues on cyclic AMP production. Prostaglandins 13: 861-871, 1977. PubMed: 194286 22898: Martin TJ, et al. Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 260: 436-438, 1976. PubMed: 1062678 22974: Ingleton PM, et al. Radiation induced osteogenic sarcoma in the rat as a model of hormone-responsive differentiated cancer. Lab. Anim. Sci. 27: 748-756, 1977. PubMed: 338978 22975: Atkins D, et al. Rat osteogenic sarcoma cells: isolation and effects of hormones on the production of cyclic AMP and cyclic GMP. Endocrinology 101: 555-562, 1977. PubMed: 195798 22989: Crawford A, et al. Rat osteogenic sarcoma cells: comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin), 6-keto F1alpha and thromboxane B2 on cyclic AMP production and adenylate cyclase activity. Biochem. Biophys. Res. Commun. 82: 1195-1201, 1978. PubMed: 212039 23013: Gallinaro BJ, et al. Activation of protein kinase C inhibits ATP-induced [Ca2+]i elevation in rat osteoblastic cells: selective effects on P2Y and P2U signaling pathways. J. Cell. Physiol. 162: 305-314, 1995. PubMed: 7860638 23381: . . Clin. Orthop. Relat. Res. 140: 247-254, 1979. 23398: Manolagas SC, et al. 1,25-Dihydroxyvitamin D3 receptor-like macromolecule in rat osteogenic sarcoma cell lines. J. Biol. Chem. 255: 4414-4417, 1980. PubMed: 6929275 23491: Underwood JC, et al. Structural and functional correlations in parathyroid hormone responsive transplantable osteogenic sarcomas. Eur. J. Cancer 15: 1151-1158, 1979. PubMed: 294355 24392: . . Trans. Biochem. Sic. 6: 239-241, 1978. 26135: Partridge NC, et al. Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. Cancer Res. 43: 4308-4315, 1983. PubMed: 6575864 26136: Martin TJ, et al. Hormonal influences on bone cells. Methods Enzymol. 145: 324-336, 1987. PubMed: 3474491 26137: Forrest SM, et al. Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin. Calcif. Tissue Int. 37: 51-56, 1985. PubMed: 3922597 33152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651

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CRL-1661 UMR-106 大鼠骨肉瘤細(xì)胞

CRL-1661 UMR-106 大鼠骨肉瘤細(xì)胞， ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！